热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
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热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号：MCHM00 规格：96T
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
The recovery of mouse Chemerin spiked to three levels throughout the range of the assay was evaluated.
Mouse Chemerin Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
3.47 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse Chemerin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Chemerin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature Chemerin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Chemerin. Results obtained using natural mouse Chemerin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Chemerin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
153
405
1124
168
385
1065
Standard Deviation
13
16.2
99
14.2
25.7
64.1
CV%
8.5
4
8.8
8.4
6.7
6
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Samples (n=4)
102
89-117
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Chemerin in each matrix were diluted with Calibrator Diluent and assayed.
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