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Human Apolipoprotein B/ApoB Quantikine ELISA Kit
Human Apolipoprotein B/ApoB Quantikine ELISA Kit
Human Apolipoprotein B/ApoB Quantikine ELISA Kit

Human Apolipoprotein B/ApoB Quantikine ELISA Kit

 详情注明

Human Apolipoprotein B/ApoB Quantikine ELISA Kit Summary

货号:DAPB00

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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
9.97 ng/mL
Assay Range
39.1 - 2,500 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural human ApoB
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Apolipoprotein B/ApoB Immunoassay is a 4.5 hour solid phase ELISA designed to measure ApoB levels in serum and plasma.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 284 654 1228 261 717 1375
Standard Deviation 15 33.6 48.1 27.4 61.2 90.5
CV% 5.3 5.1 3.9 10.5 8.5 6.6

Linearity

To assess the linearity of the assay, samples containing high concentrations of ApoB were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Apolipoprotein B/ApoB ELISA Linearity

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