热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
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热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号:MCX900 规格：96T
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma
The recovery of mouse MIG spiked to three levels throughout the range of the assay in various matrices was evaluated.
Mouse CXCL9/MIG Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
Sensitivity
7.8 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse MIG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIG in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIG and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIG.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
23
23
23
Mean
77
216
815
66
190
741
Standard Deviation
6.2
14.8
68
5.5
14.1
39.5
CV%
8.1
6.9
8.3
8.3
7.4
5.3
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=6)
95
81-116
EDTA Plasma (n=9)
94
81-107
Serum (n=9)
93
85-112
Tissue Homogenates (n=2)
104
88-118
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MIG in each matrix were diluted with Calibrator Diluent and then assayed.
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