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热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号：DAR00
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
The recovery of Amphiregulin spiked to levels throughout the range of the assay in various matrices was evaluated.
Human Amphiregulin Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
Sensitivity
3.56 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Recombinant and natural human Amphiregulin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Amphiregulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Amphiregulin in cell culture supernates, cell lysates serum, plasma, saliva, and urine. It contains E. coli-derived recombinant human Amphiregulin and has been shown to accurately quantitate the recombinant factor. Results obtained with natural human Amphiregulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amphiregulin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration
were tested in separate assays to assess inter-assay precision. Assays
were performed by at least three technicians using two lots of
components
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
102
314
631
99.4
308
614
Standard Deviation
5.63
10.5
22.4
9.85
20.5
28.8
CV%
5.5
3.3
3.5
9.9
6.7
4.7
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
95
88-101
EDTA Plasma (n=4)
93
82-105
Heparin Plasma (n=4)
94
82-103
Saliva (n=4)
104
98-113
Serum (n=4)
95
85-103
Urine (n=4)
88
75-99
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Amphiregulin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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